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Continuous pH monitoring in a perfused bioreactor system using an optical pH sensorMonitoring and regulating the pH of the solution in a bioprocess is one of the key steps in the success of bioreactor operation. An in-line optical pH sensor, based on the optical absorption properties of phenol red present in the medium, was developed and tested in this work for use in NASA space bioreactors based on a rotating wall-perfused vessel system supporting a baby hamster kidney (BHK-21) cell culture. The sensor was tested over three 30-day and one 124-day cell runs. The pH sensor initially was calibrated and then used during the entire cell culture interval. The pH reported by the sensor was compared to that measured by a fiber optically coupled Shimadzu spectrophotometer and a blood gas analyzer. The maximum standard error of prediction for all the four cell runs for development pH sensor against BGA was +/-0.06 pH unit and for the fiber optically coupled Shimadzu spectrophotometer against the blood gas analyzer was +/-0.05 pH unit. The pH sensor system performed well without need of recalibration for 124 days. Copyright 2002 Wiley Periodicals, Inc.
Document ID
20040088384
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Jeevarajan, Antony S. (Life Sciences-Systems and Services 1290 Hercules Drive, Suite 120, Mail Stop BT-37, Houston, Texas 77058, United States)
Vani, Sundeep
Taylor, Thomas D.
Anderson, Melody M.
Date Acquired
August 21, 2013
Publication Date
May 20, 2002
Publication Information
Publication: Biotechnology and bioengineering
Volume: 78
Issue: 4
ISSN: 0006-3592
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other