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Interactions involving the Rad51 paralogs Rad51C and XRCC3 in human cellsHomologous recombinational repair of DNA double-strand breaks and crosslinks in human cells is likely to require Rad51 and the five Rad51 paralogs (XRCC2, XRCC3, Rad51B/Rad51L1, Rad51C/Rad51L2 and Rad51D/Rad51L3), as has been shown in chicken and rodent cells. Previously, we reported on the interactions among these proteins using baculovirus and two- and three-hybrid yeast systems. To test for interactions involving XRCC3 and Rad51C, stable human cell lines have been isolated that express (His)6-tagged versions of XRCC3 or Rad51C. Ni2+-binding experiments demonstrate that XRCC3 and Rad51C interact in human cells. In addition, we find that Rad51C, but not XRCC3, interacts directly or indirectly with Rad51B, Rad51D and XRCC2. These results argue that there are at least two complexes of Rad51 paralogs in human cells (Rad51C-XRCC3 and Rad51B-Rad51C-Rad51D-XRCC2), both containing Rad51C. Moreover, Rad51 is not found in these complexes. X-ray treatment did not alter either the level of any Rad51 paralog or the observed interactions between paralogs. However, the endogenous level of Rad51C is moderately elevated in the XRCC3-overexpressing cell line, suggesting that dimerization between these proteins might help stabilize Rad51C.
Document ID
20040088470
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Wiese, Claudia
(Lawrence Berkeley National Laboratory Berkeley, CA 94720, United States)
Collins, David W.
Albala, Joanna S.
Thompson, Larry H.
Kronenberg, Amy
Schild, David
Chatterjee, A.
Date Acquired
August 21, 2013
Publication Date
February 15, 2002
Publication Information
Publication: Nucleic acids research
Volume: 30
Issue: 4
ISSN: 0305-1048
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: CA81019
CONTRACT_GRANT: GM30990
CONTRACT_GRANT: CA73966
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Radiation Health

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