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Enhanced animal growth via ligand-regulated GHRH myogenic-injectable vectorsRegulated animal growth occurred following a single electroporated injection of a mixture of two plasmids (10 microg of DNA), one expressing the GeneSwitch regulator protein, the other an inducible growth hormone releasing hormone (GHRH) gene, into the tibialis anterior muscles of adult SCID mice. Administration of the ligand mifepristone (MFP) up-regulated GHRH expression, as shown by elevations of IGF-I levels, and when MFP dosing was withdrawn, IGF-I returned to baseline levels. Five cycles of IGF-I induction were observed during a five-month period. Chronic MFP dosing for 25 days increased lean body mass, weight gain, and bone mineral density significantly compared with non-MFP treated controls. In summary, long-term drug-regulated GHRH expression was achieved following plasmid-based gene therapy, and chronic induction of GHRH expression in adult animals led to improvements in weight gain and body composition.
Document ID
20040088524
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Draghia-Akli, Ruxandra
(ASR-USDA Baylor College of Medicine Houston, Texas 77030, United States)
Malone, P. Brandon
Hill, Leigh Anne
Ellis, Kenneth M.
Schwartz, Robert J.
Nordstrom, Jeffrey L.
Date Acquired
August 21, 2013
Publication Date
March 1, 2002
Publication Information
Publication: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume: 16
Issue: 3
ISSN: 0892-6638
Subject Category
Aerospace Medicine
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Musculoskeletal
Non-NASA Center

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