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Genomic instability in human lymphoid cells exposed to 1 GeV/amu Fe ionsThe goal of this study was to assess whether charged particle radiations of importance to spaceflight elicit genomic instability in human TK6 lymphoblasts. The incidence of genomic instability in TK6 cells was assessed ~21 days after exposure to 2, 4, or 6 Fe ions (1 GeV/amu, LET= 146 keV/micrometers). Three indices of instability were used: intraclonal karyotypic heterogeneity, mutation rate analysis at the thymidine kinase (TK1) locus, and re-cloning efficiency. Fifteen of sixty clones demonstrated karyotypic heterogeneity. Five clones had multiple indicators of karyotypic change. One clone was markedly hypomutable and polyploid. Six clones were hypomutable, while 21 clones were mutators. Of these, seven were karyotypically unstable. Six clones had low re-cloning efficiencies, one of which was a mutator. All had normal karyotypes. In summary, many clones that survived exposure to a low fluence of Fe ions manifested one or more forms of genomic instability that may hasten the development of neoplasia through deletion or by recombination.
Document ID
20040088533
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Grosovsky, A.
(Univ. California Riverside Riverside, CA, United States)
Bethel, H.
Parks, K.
Ritter, L.
Giver, C.
Gauny, S.
Wiese, C.
Kronenberg, A.
Date Acquired
August 21, 2013
Publication Date
January 1, 2001
Publication Information
Publication: Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)
Volume: 17 Suppl 1
ISSN: 1120-1797
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: CA 73966
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Radiation Health

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