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Record Details

Record 61 of 1184
Endothelial NOS-dependent activation of c-Jun NH(2)- terminal kinase by oxidized low-density lipoprotein
Author and Affiliation:
Go, Y. M.(University of Alabama at Birmingham, Department of Pathology, Birmingham, Alabama 35294, United States)
Levonen, A. L.
Moellering, D.
Ramachandran, A.
Patel, R. P.
Jo, H.
Darley-Usmar, V. M.
Abstract: Oxidized low-density lipoprotein (oxLDL) is known to activate a number of signal transduction pathways in endothelial cells. Among these are the c-Jun NH(2)-terminal kinase (JNK), also known as stress-activated protein kinase, and extracellular signal-regulated kinase (ERK). These mitogen-activated protein kinases (MAP kinase) determine cell survival in response to environmental stress. Interestingly, JNK signaling involves redox-sensitive mechanisms and is activated by reactive oxygen and nitrogen species derived from both NADPH oxidases, nitric oxide synthases (NOS), peroxides, and oxidized low-density lipoprotein (oxLDL). The role of endothelial NOS (eNOS) in the activation of JNK in response to oxLDL has not been examined. Herein, we show that on exposure of endothelial cells to oxLDL, both ERK and JNK are activated through independent signal transduction pathways. A key role of eNOS activation through a phosphatidylinositol-3-kinase-dependent mechanism leading to phosphorylation of eNOS is demonstrated for oxLDL-dependent activation of JNK. Moreover, we show that activation of ERK by oxLDL is critical in protection against the cytotoxicity of oxLDL.
Publication Date: Dec 01, 2001
Document ID:
20040088651
(Acquired Sep 07, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: American journal of physiology. Heart and circulatory physiology (ISSN 0363-6135); Volume 281; 6; H2705-13
Publisher Information: United States
Contract/Grant/Task Num: ES; HL-58031; HL-60905
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: ENZYMES; LIPOPROTEINS; AORTA; CATTLE; CULTURED CELLS; CYTOLOGY; ENDOTHELIUM; ENZYME ACTIVITY; ENZYME INHIBITORS; NITRIC OXIDE; ONCOGENES; PROTEINS
Other Descriptors: LIPOPROTEINS, LDL/TOXICITY; MITOGEN-ACTIVATED PROTEIN KINASES/ANTAGONISTS & INHIBITORS/METABOLISM; NITRIC-OXIDE SYNTHASE/METABOLISM; ANIMALS; AORTA, THORACIC/CYTOLOGY; CATTLE; CELLS, CULTURED; ENDOTHELIUM, VASCULAR/CYTOLOGY/DRUG EFFECTS/ENZYMOLOGY; ENZYME ACTIVATION/DRUG EFFECTS/PHYSIOLOGY; ENZYME INHIBITORS/PHARMACOLOGY; FLAVONOIDS/PHARMACOLOGY; HUMAN; MAP KINASE SIGNALING SYSTEM/DRUG EFFECTS/PHYSIOLOGY; NADPH OXIDASE/METABOLISM; NITRIC OXIDE/METABOLISM; PROTO-ONCOGENE PROTEINS/METABOLISM; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S; SUPPORT, U.S. GOV'T, P.H.S; NASA DISCIPLINE CELL BIOLOGY; NON-NASA CENTER
Availability Source: Other Sources
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