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Expression of pH-sensitive green fluorescent protein in Arabidopsis thalianaThis is the first report on using green fluorescent protein (GFP) as a pH reporter in plants. Proton fluxes and pH regulation play important roles in plant cellular activity and therefore, it would be extremely helpful to have a plant gene reporter system for rapid, non-invasive visualization of intracellular pH changes. In order to develop such a system, we constructed three vectors for transient and stable transformation of plant cells with a pH-sensitive derivative of green fluorescent protein. Using these vectors, transgenic Arabidopsis thaliana and tobacco plants were produced. Here the application of pH-sensitive GFP technology in plants is described and, for the first time, the visualization of pH gradients between different developmental compartments in intact whole-root tissues of A. thaliana is reported. The utility of pH-sensitive GFP in revealing rapid, environmentally induced changes in cytoplasmic pH in roots is also demonstrated.
Document ID
Document Type
Reprint (Version printed in journal)
Moseyko, N. (University of California at Berkeley Berkeley, CA 94720-3102, United States)
Feldman, L. J.
Date Acquired
August 21, 2013
Publication Date
May 1, 2001
Publication Information
Publication: Plant, cell & environment
Volume: 24
Issue: 5
ISSN: 0140-7791
Subject Category
Life Sciences (General)
Distribution Limits
NASA Discipline Plant Biology
Non-NASA Center