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Ultrastructural analyses of somatic embryo initiation, development and polarity establishment from mesophyll cells of Dactylis glomerataSomatic embryos initiate and develop directly from single mesophyll cells in in vitro-cultured leaf segments of orchardgrass (Dactylis glomerata L.). Embryogenic cells establish themselves in the predivision stage by formation of thicker cell walls and dense cytoplasm. Electron microscopy observations for embryos ranging from the pre-cell-division stage to 20-cell proembryos confirm previous light microscopy studies showing a single cell origin. They also confirm that the first division is predominantly periclinal and that this division plane is important in establishing embryo polarity and in determining the embryo axis. If the first division is anticlinal or if divisions are in random planes after the first division, divisions may not continue to produce an embryo. This result may produce an embryogenic cell mass, callus formation, or no structure at all. Grant numbers: NAGW-3141, NAG10-0221.
Document ID
20040088680
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Vasilenko, A.
(The University of Tennessee Knoxville, Tennessee 37901-1071, United States)
McDaniel, J. K.
Conger, B. V.
Date Acquired
August 21, 2013
Publication Date
January 1, 2000
Publication Information
Publication: In vitro cellular & developmental biology. Plant : journal of the Tissue Culture Association
Volume: 36
Issue: 1
ISSN: 1054-5476
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Plant Biology

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