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Record Details

Record 38 of 2660
Phase sensitive x-ray diffraction imaging of defects in biological macromolecular crystals
External Online Source: doi:10.1103/PhysRevLett.87.148101
Author and Affiliation:
Hu, Z. W.(NASA Marshall Space Flight Center, Huntsville AL United States)
Lai, B.
Chu, Y. S.
Cai, Z.
Mancini, D. C.
Thomas, B. R.
Chernov, A. A.
Abstract: Conventional x-ray diffraction topography is currently used to map defects in the bulk of protein crystals, but the lack of sufficient contrast is frequently a limiting factor. We experimentally demonstrate that this barrier can be circumvented using a method that combines phase sensitive and diffraction imaging principles. Details of defects revealed in tetragonal lysozyme and cubic ferritin crystals are presented and discussed. The approach enabling the detection of the phase changes of diffracted x rays should prove to be useful in the study of defect structures in a broad range of biological macromolecular crystals.
Publication Date: Oct 01, 2001
Document ID:
20040088748
(Acquired Sep 07, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Physical review letters (ISSN 0031-9007); Volume 87; 14; 148101
Publisher Information: United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: CRYSTALS; DEFECTS; IMAGING TECHNIQUES; PROTEINS; SENSITIVITY; X RAY DIFFRACTION; CHICKENS; CRYSTALLIZATION; FEMALES
Other Descriptors: FERRITIN/CHEMISTRY; MURAMIDASE/CHEMISTRY; ANIMALS; CHICKENS; CRYSTALLIZATION; EGG PROTEINS/CHEMISTRY; FEMALE; X-RAY DIFFRACTION
Availability Source: Other Sources
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