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Record 2 of 200
Decreased non-MHC-restricted (CD56+) killer cell cytotoxicity after spaceflight
Author and Affiliation:
Mehta, S. K.(Enterprise Advisory Services Inc., Houston, TX 77058, United States)
Kaur, I.
Grimm, E. A.
Smid, C.
Feeback, D. L.
Pierson, D. L.
Abstract: Cytotoxic activity of non-major histocompatibility complex-restricted (CD56+) (NMHC) killer cells and cell surface marker expression of peripheral blood mononuclear cells were determined before and after spaceflight. Ten astronauts (9 men, 1 woman) from two space shuttle missions (9- and 10-day duration) participated in the study. Blood samples were collected 10 days before launch, within 3 h after landing, and 3 days after landing. All peripheral blood mononuclear cell preparations were cryopreserved and analyzed simultaneously in a 4-h cytotoxicity (51)Cr release assay using K562 target cells. NMHC killer cell lytic activity was normalized per 1,000 CD56+ cells. When all 10 subjects were considered as one study group, NMHC killer cell numbers did not change significantly during the three sampling periods, but at landing lytic activity had decreased by approximately 40% (P < 0.05) from preflight values. Nine of ten astronauts had decreased lytic activity immediately after flight. NMHC killer cell cytotoxicity of only three astronauts returned toward preflight values by 3 days after landing. Consistent with decreased NMHC killer cell cytotoxicity, urinary cortisol significantly increased after landing compared with preflight levels. Plasma cortisol and ACTH levels at landing were not significantly different from preflight values. No correlation of changes in NMHC killer cell function or hormone levels with factors such as age, gender, mission, or spaceflight experience was found. After landing, expression of the major lymphocyte surface markers (CD3, CD4, CD8, CD14, CD16, CD56), as determined by flow cytometric analysis, did not show any consistent changes from measurements made before flight.
Publication Date: Oct 01, 2001
Document ID:
20040088755
(Acquired Sep 07, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Journal of applied physiology (Bethesda, Md. : 1985) (ISSN 8750-7587); Volume 91; 4; 1814-8
Publisher Information: United States
Financial Sponsor: NASA Johnson Space Center; Houston TX United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: ANTIGENS; IMMUNITY; LYMPHOCYTES; SPACE FLIGHT; ADULTS; CHROMIUM ISOTOPES; CRYOGENIC COOLING; CYTOMETRY; FEMALES; HYDROXYCORTICOSTEROID; MALES; PITUITARY HORMONES; PRESERVING; RADIOACTIVE ISOTOPES; SPACE SHUTTLE MISSIONS
Other Descriptors: ANTIGENS, CD56/IMMUNOLOGY; IMMUNITY, CELLULAR/PHYSIOLOGY; KILLER CELLS, NATURAL/IMMUNOLOGY; SPACE FLIGHT; ADULT; ANTIGENS, SURFACE/ANALYSIS; CHROMIUM RADIOISOTOPES/DIAGNOSTIC USE; CORTICOTROPIN/BLOOD; CRYOPRESERVATION; FEMALE; FLOW CYTOMETRY; HUMAN; HYDROCORTISONE/BLOOD; MAJOR HISTOCOMPATIBILITY COMPLEX/IMMUNOLOGY; MALE; MIDDLE AGED; SUPPORT, U.S. GOV'T, NON-P.H.S; FLIGHT EXPERIMENT; STS SHUTTLE PROJECT; MANNED; SHORT DURATION; CLINICAL TRIAL; NASA CENTER JSC; NASA DISCIPLINE ENVIRONMENTAL HEALTH
Availability Source: Other Sources
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