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Record Details

Record 26 of 1683
Sequence dependent N-terminal rearrangement and degradation of peptide nucleic acid (PNA) in aqueous solution
Author and Affiliation:
Eriksson, M.(The Panum Institute, Dept. of Biochemistry B, Copenhagen, Denmark)
Christensen, L.
Schmidt, J.
Haaima, G.
Orgel, L.
Nielsen, P. E.
Abstract: The stability of the PNA (peptide nucleic acid) thymine monomer inverted question markN-[2-(thymin-1-ylacetyl)]-N-(2-aminoaminoethyl)glycine inverted question mark and those of various PNA oligomers (5-8-mers) have been measured at room temperature (20 degrees C) as a function of pH. The thymine monomer undergoes N-acyl transfer rearrangement with a half-life of 34 days at pH 11 as analyzed by 1H NMR; and two reactions, the N-acyl transfer and a sequential degradation, are found by HPLC analysis to occur at measurable rates for the oligomers at pH 9 or above. Dependent on the amino-terminal sequence, half-lives of 350 h to 163 days were found at pH 9. At pH 12 the half-lives ranged from 1.5 h to 21 days. The results are discussed in terms of PNA as a gene therapeutic drug as well as a possible prebiotic genetic material.
Publication Date: Jan 01, 1998
Document ID:
20040088776
(Acquired Sep 07, 2004)
Subject Category: EXOBIOLOGY
Document Type: Journal Article
Publication Information: Nouveau journal de chimie (ISSN 0398-9836); Volume 22; 1055-9
Publisher Information: France
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: AQUEOUS SOLUTIONS; CHEMICAL EVOLUTION; DEGRADATION; NUCLEIC ACIDS; PEPTIDES; POLYMERS; THYMINE; WATER; EXOBIOLOGY; ION CONCENTRATION; ISOMERS; MAGNETIC RESONANCE SPECTROSCOPY; SOLUTIONS
Other Descriptors: EVOLUTION, CHEMICAL; PEPTIDE NUCLEIC ACIDS/CHEMICAL SYNTHESIS/CHEMISTRY; POLYMERS/CHEMICAL SYNTHESIS/CHEMISTRY; THYMINE/ANALOGS & DERIVATIVES; WATER/CHEMISTRY; HYDROGEN-ION CONCENTRATION; ISOMERISM; MAGNETIC RESONANCE SPECTROSCOPY; SOLUTIONS; NASA DISCIPLINE EXOBIOLOGY; NON-NASA CENTER
Availability Source: Other Sources
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