NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Immunolocalization of integrin-like proteins in Arabidopsis and CharaIntegrins are a large family of integral plasma membrane proteins that link the extracellular matrix to the cytoskeleton in animal cells. As a first step in determining if integrin-like proteins are involved in gravitropic signal transduction pathways, we have used a polyclonal antibody against the chicken beta1 integrin subunit in western blot analyses and immunofluorescence microscopy to gain information on the size and location of these proteins in plants. Several different polypeptides are recognized by the anti-integrin antibody in roots and shoots of Arabidopsis and in the internodal cells and rhizoids of Chara. These cross-reactive polypeptides are associated with cellular membranes, a feature which is consistent with the known location of integrins in animal systems. In immunofluorescence studies of Arabidopsis roots, a strong signal was obtained from labeling integrin-like proteins in root cap cells, and there was little or no immunolabel in other regions of the root tip. While the antibody stained throughout Chara rhizoids, the highest density of immunolabel was at the tip. Thus, in both Arabidopsis roots and Chara rhizoids, the sites of gravity perception/transduction appear to be enriched in integrin-like molecules.
Document ID
20040088823
Acquisition Source
Ames Research Center
Document Type
Reprint (Version printed in journal)
Authors
Katembe, W. J.
(Miami Univ. Oxford, OH 45056, United States)
Swatzell, L. J.
Makaroff, C. A.
Kiss, J. Z.
Date Acquired
August 21, 2013
Publication Date
January 1, 1997
Publication Information
Publication: Physiologia plantarum
Volume: 99
Issue: 1
ISSN: 0031-9317
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAG2-1017
CONTRACT_GRANT: NAGW-3783
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Plant Biology

Available Downloads

There are no available downloads for this record.
No Preview Available