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Diversity and regulation of plant Ca2+ pumps: insights from expression in yeastThe spatial and temporal regulation of calcium concentration in plant cells depends on the coordinate activities of channels and active transporters located on different organelles and membranes. Several Ca2+ pumps have been identified and characterized by functional expression of plant genes in a yeast mutant (K616). This expression system has opened the way to a genetic and biochemical characterization of the regulatory and catalytic features of diverse Ca2+ pumps. Plant Ca(2+)-ATPases fall into two major types: AtECA1 represents one of four or more members of the type IIA (ER-type) Ca(2+)-ATPases in Arabidopsis, and AtACA2 is one of seven or more members of the type IIB (PM-type) Ca(2+)-ATPases that are regulated by a novel amino terminal domain. Type IIB pumps are widely distributed on membranes, including the PM (plasma membrane), vacuole, and ER (endoplasmic reticulum). The regulatory domain serves multiple functions, including autoinhibition, calmodulin binding, and sites for modification by phosphorylation. This domain, however, is considerably diverse among several type IIB ATPases, suggesting that the pumps are differentially regulated. Understanding of Ca2+ transporters at the molecular level is providing insights into their roles in signaling networks and in regulating fundamental processes of cell biology.
Document ID
Document Type
Reprint (Version printed in journal)
Sze, H.
(College Park 20742 United States)
Liang, F.
Hwang, I.
Curran, A. C.
Harper, J. F.
Evans, M. L.
Date Acquired
August 21, 2013
Publication Date
January 1, 2000
Publication Information
Publication: Annual review of plant physiology and plant molecular biology
Volume: 51
ISSN: 1040-2519
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: 9735304-4523
Distribution Limits
Review, Academic
Non-NASA Center
NASA Discipline Plant Biology

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