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Phytochrome regulates GTP-binding protein activity in the envelope of pea nucleiThree GTP-binding proteins with apparent molecular masses of 27, 28 and 30 kDa have been detected in isolated nuclei of etiolated pea plumules. After LDS-PAGE and transfer to nitrocellulose these proteins bind [32P]GTP in the presence of excess ATP, suggesting that they are monomeric G proteins. When nuclei are disrupted, three proteins co-purify with the nuclear envelope fraction and are highly enriched in this fraction. The level of [32P]GTP-binding for all three protein bands is significantly increased when harvested pea plumules are irradiated by red light, and this effect is reversed by far-red light. The results indicate that GTP-binding activity associated with the nuclear envelope of plant cells is photoreversibly regulated by the pigment phytochrome.
Document ID
20040088990
Document Type
Reprint (Version printed in journal)
Authors
Clark, G. B. (University of Texas Austin 78713, United States)
Memon, A. R.
Thompson, G. A. Jr
Roux, S. J.
Date Acquired
August 21, 2013
Publication Date
January 1, 1993
Publication Information
Publication: The Plant journal : for cell and molecular biology
Volume: 4
Issue: 2
ISSN: 0960-7412
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAGW-1519
CONTRACT_GRANT: DMB-88-2838
CONTRACT_GRANT: DCB-9106245
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Plant Biology
NASA Discipline Number 40-50
NASA Program Space Biology
Non-NASA Center