NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Expression of spicule matrix protein gene SM30 in embryonic and adult mineralized tissues of sea urchin Hemicentrotus pulcherrimusWe have isolated a cDNA clone for spicule matrix protein, SM30, from sea urchin Hemicentrotus pulcherrimus and have studied the expression of this gene in comparison with that of another spicule matrix protein gene, SM50. In cultured micromeres as well as in intact embryos transcripts of SM30 were first detectable around the onset of spicule formation and rapidly increased with the growth of spicules, which accompanied accumulation of glycosylated SM30 protein(s). When micromeres were cultured in the presence of Zn2+, spicule formation and SM30 expression were suppressed, while both events resumed concurrently after the removal of Zn2+ from the culture medium. Expression of SM50, in contrast, started before the appearance of spicules and was not sensitive to Zn2+. Differences were also observed in adult tissues; SM30 mRNA was detected in spines and tube feet but not in the test, while SM50 mRNA was apparent in all of these mineralized tissues at similar levels. These results strongly suggest that the SM30 gene is regulated by a different mechanism to that of the SM50 gene and that the products of these two genes are differently involved in sea urchin biomineralization. A possible role of SM30 protein in skeleton formation is discussed.
Document ID
20040089143
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Kitajima, T.
(Tokyo Metropolitan University Tokyo)
Tomita, M.
Killian, C. E.
Akasaka, K.
Wilt, F. H.
Date Acquired
August 21, 2013
Publication Date
December 1, 1996
Publication Information
Publication: Development, growth & differentiation
Volume: 38
Issue: 6
ISSN: 0012-1592
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Musculoskeletal
Non-NASA Center

Available Downloads

There are no available downloads for this record.
No Preview Available