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Protein expression in Arabidopsis thaliana after chronic clinorotationSoluble protein expression in Arabidopsis thaliana L. (Heynh.) leaf and stem tissue was examined after chronic clinorotation. Seeds of Arabidopsis were germinated and plants grown to maturity on horizontal or vertical slow-rotating clinostats (1 rpm) or in stationary vertical control units. Total soluble proteins and in vivo-labeled soluble proteins isolated from these plants were analyzed by two-dimensional SDS PAGE and subsequent fluorography. Visual and computer analysis of the resulting protein patterns showed no significant differences in either total protein expression or in active protein synthesis between horizontal clinorotation and vertical controls in the Arabidopsis leaf and stem tissue. These results show chronic clinorotation does not cause gross changes in protein expression in Arabidopsis.
Document ID
20040089172
Acquisition Source
Kennedy Space Center
Document Type
Reprint (Version printed in journal)
Authors
Piastuch, W. C.
(NASA Kennedy Space Center Cocoa Beach FL United States)
Brown, C. S.
Date Acquired
August 21, 2013
Publication Date
June 1, 1995
Publication Information
Publication: Journal of plant physiology
Volume: 146
Issue: 3
ISSN: 0176-1617
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAS10-11624
Distribution Limits
Public
Copyright
Other
Keywords
NASA Center KSC
NASA Discipline Plant Biology

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