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The influence of altered gravity on carbohydrate metabolism in excised wheat leavesWe developed a system to study the influence of altered gravity on carbohydrate metabolism in excised wheat leaves by means of clinorotation. The use of excised leaves in our clinostat studies offered a number of advantages over the use of whole plants, most important of which were minimization of exogenous mechanical stress and a greater amount of carbohydrate accumulation during the time of treatment. We found that horizontal clinorotation of excised wheat leaves resulted in significant reductions in the accumulation of fructose, sucrose, starch and fructan relative to control, vertically clinorotated leaves. Photosynthesis, dark respiration and the extractable activities of ADP glucose pyrophosphorylase (EC 2.7.7.27), sucrose phosphate synthase (EC 2.4.4.14), sucrose sucrose fructosyltransferase (EC 2.4.1.99), and fructan hydrolase (EC 3.2.1.80) were unchanged due to altered gravity treatment.
Document ID
20040089174
Document Type
Reprint (Version printed in journal)
Authors
Obenland, D. M.
(NASA Kennedy Space Center Cocoa Beach FL United States)
Brown, C. S.
Date Acquired
August 21, 2013
Publication Date
November 1, 1994
Publication Information
Publication: Journal of plant physiology
Volume: 144
Issue: 6
ISSN: 0176-1617
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Center KSC
NASA Discipline Plant Biology
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