NASA Logo, External Link
Facebook icon, External Link to NASA STI page on Facebook Twitter icon, External Link to NASA STI on Twitter YouTube icon, External Link to NASA STI Channel on YouTube RSS icon, External Link to New NASA STI RSS Feed AddThis share icon
 

Record Details

Record 19 of 2596
Radiogenic transformation of human mammary epithelial cells in vitro
Author and Affiliation:
Yang, T. C.(NASA Johnson Space Center, Houston TX United States)
Georgy, K. A.
Tavakoli, A.
Craise, L. M.
Durante, M.
Abstract: Cancer induction by space radiations is a major concern for manned space exploration. Accurate assessment of radiation risk at low doses requires basic understanding of mechanism(s) of radiation carcinogenesis. For determining the oncogenic effects of ionizing radiation in human epithelial cells, we transformed a mammary epithelial cell line (185B5), which was immortalized by benzo(a)pyrene, with energetic heavy ions and obtained several transformed clones. These transformed cells showed growth properties on Matrigel similar to human mammary tumor cells. To better understand the mechanisms of radiogenic transformation of human cells, we systematically examined the alterations in chromosomes and cancer genes. Among 16 autosomes examined for translocations, by using fluorescence in situ hybridization (FISH) technique, chromosomes 3, 12, 13, 15, 16, and 18 appeared to be normal in transformed cells. Chromosomes 1, 4, 6, 8, and 17 in transformed cells, however, showed patterns different from those in nontransformed cells. Southern blot analyses indicated no detectable alterations in myc, ras, Rb, or p53 genes. Further studies of chromosome 17 by using in situ hybridization with unique sequence p53 gene probe and a centromere probe showed no loss of p53 gene in transformed cells. Experimental results from cell fusion studies indicated that the transforming gene(s) is recessive. The role of genomic instability and tumor suppressor gene(s) in radiogenic transformation of human breast cells remains to be identified.
Publication Date: Jan 01, 1996
Document ID:
20040089220
(Acquired Sep 07, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Radiation oncology investigations (ISSN 1065-7541); Volume 3; 412-9
Publisher Information: United States
Financial Sponsor: NASA Johnson Space Center; Houston TX United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: BREAST; COSMIC RAYS; IN VITRO METHODS AND TESTS; LINEAR ENERGY TRANSFER (LET); MAMMARY GLANDS; NEOPLASMS; RADIOGENIC MATERIALS; CHROMOSOME ABERRATIONS; CHROMOSOMES; CULTURED CELLS; DEOXYRIBONUCLEIC ACID; EPITHELIUM; FEMALES; GENES; GENETICS; HEAVY IONS; PARTICLE ACCELERATORS; PATHOLOGY; RADIATION EFFECTS; TUMOR SUPPRESSOR GENES
Other Descriptors: BREAST/PATHOLOGY/RADIATION EFFECTS; CELL TRANSFORMATION, NEOPLASTIC; COSMIC RADIATION; LINEAR ENERGY TRANSFER; CELL FUSION; CELL LINE, TRANSFORMED; CHROMOSOME ABERRATIONS; CHROMOSOMES/GENETICS/RADIATION EFFECTS; DNA/GENETICS/RADIATION EFFECTS; EPITHELIUM/PATHOLOGY/RADIATION EFFECTS; FEMALE; GENES, RECESSIVE/GENETICS; GENES, TUMOR SUPPRESSOR/GENETICS; HEAVY IONS; HUMAN; IN SITU HYBRIDIZATION, FLUORESCENCE; KARYOTYPING; PARTICLE ACCELERATORS; SUPPORT, U.S. GOV'T, NON-P.H.S; NASA CENTER JSC; NASA DISCIPLINE RADIATION HEALTH
Availability Source: Other Sources
› Back to Top
NASA Logo, External Link
NASA Official: Gerald Steeman
Site Curator: STI Program
Last Modified: August 23, 2011
Contact Us