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Record Details

Record 27 of 644
Mechanotransduction molecules in the plant gravisensory response: amyloplast/statolith membranes contain a beta 1 integrin-like protein
External Online Source: doi:10.1007/BF01280715
Author and Affiliation:
Lynch, T. M.(University of Vermont, Botany Department, Burlington, United States)
Lintilhac, P. M.
Domozych, D.
Abstract: It has been hypothesized that the sedimentation of amyloplasts within root cap cells is the primary event in the plant gravisensory-signal transduction cascade. Statolith sedimentation, with its ability to generate weighty mechanical signals, is a legitimate means for organisms to discriminate the direction of the gravity vector. However, it has been demonstrated that starchless mutants with reduced statolith densities maintain some ability to sense gravity, calling into question the statolith sedimentation hypothesis. Here we report on the presence of a beta 1 integrin-like protein localized inside amyloplasts of tobacco NT-1 suspension culture, callus cells, and whole-root caps. Two different antibodies to the beta 1 integrin, one to the cytoplasmic domain and one to the extracellular domain, localize in the vicinity of the starch grains within amyloplasts of NT-1. Biochemical data reveals a 110-kDa protein immunoprecipitated from membrane fractions of NT-1 suspension culture indicating size homology to known beta 1 integrin in animals. This study provides the first direct evidence for the possibility of integrin-mediated signal transduction in the perception of gravity by higher plants. An integrin-mediated pathway, initiated by starch grain sedimentation within the amyloplast, may provide the signal amplification necessary to explain the gravitropic response in starch-depleted cultivars.
Publication Date: Jan 01, 1998
Document ID:
20040089258
(Acquired Sep 07, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Protoplasma; p. 92-100; (ISSN 0033-183X); Volume 201; 1-2
Publisher Information: Austria
Contract/Grant/Task Num: NAGW-3604
Financial Sponsor: NASA; Washington DC United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: ANTIGENS; GRAVITY PERCEPTION; MEMBRANES; MOLECULES; PLANTS (BOTANY); PLASTIDS; PROTEINS; SIGNAL TRANSMISSION; CYTOLOGY; ELECTRON MICROSCOPY; EXOBIOLOGY; PLANT ROOTS; STARCHES; TOBACCO; TOXINS AND ANTITOXINS
Other Descriptors: ANTIGENS, CD29/ANALYSIS; GRAVITY PERCEPTION/PHYSIOLOGY; PLANT PROTEINS/ANALYSIS; PLASTIDS/CHEMISTRY/PHYSIOLOGY/ULTRASTRUCTURE; SIGNAL TRANSDUCTION/PHYSIOLOGY; INTEGRINS/ANALYSIS; MICROSCOPY, ELECTRON; PLANT ROOT CAP/CHEMISTRY/CYTOLOGY/PHYSIOLOGY/ULTRASTRUCTURE; PLANTS, TOXIC; STARCH; SUPPORT, U.S. GOV'T, NON-P.H.S; TOBACCO/CHEMISTRY/CYTOLOGY/PHYSIOLOGY/ULTRASTRUCTURE; NASA DISCIPLINE PLANT BIOLOGY; NON-NASA CENTER
Availability Source: Other Sources
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