NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Comparison of developmental gradients for growth, ATPase, and fusicoccin-binding activity in mung bean hypocotylsA comparison has been made of the developmental gradients along a mung bean (Vigna radiata L.) hypocotyl of the growth rate, plasma membrane ATPase, and fusicoccin-binding protein (FCBP) activity to determine whether they are interrelated. The hook and four sequential 7.5 millimeter segments of the hypocotyl below the hook were cut. A plasma membrane-enriched fraction was isolated from each section by aqueous two-phase partitioning and assayed for vanadate-sensitive ATPase and FCBP activity. Each gradient had a distinctive and different pattern. Endogenous growth rate was maximal in the second section and much lower in the others. Vanadate-sensitive ATPase activity was maximal in the third section, but remained high in the older sections. Amounts of ATPase protein, shown by specific antibody binding, did not correlate with the amount of vanadate-sensitive ATPase activity in the three youngest sections. FCBP activity was almost absent in the first section, then increased to a maximum in the oldest sections. These data show that the growth rate is not determined by the ATPase activity, and that there are no fixed ratios between the ATPase and FCBP.
Document ID
20040089327
Document Type
Reprint (Version printed in journal)
Authors
Basel, L. E.
(University of Washington Seattle 98195, United States)
Cleland, R. E.
Date Acquired
August 21, 2013
Publication Date
March 1, 1992
Publication Information
Publication: Plant physiology
Volume: 98
Issue: 3
ISSN: 0032-0889
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: DCB-8904093
CONTRACT_GRANT: 2 T32 GMO7270
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Plant Biology
No Preview Available