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SEM visualization of glycosylated surface molecules using lectin-coated microspheresThere are several techniques currently used to localize glycosylated surface molecules by scanning electron microscopy (Grinnell, 1980; Molday, 1976; Linthicum and Sell, 1975; Nicolson, 1974; Lo Buglio, et al, 1972). A simple and rapid method, using a modification of Grinnell's technique is reported here. Essentially, microspheres coated with Concavalin A are used to bind to glycosylated regions of the palatal shelf epithelium and are visualized in the scanning electron microscope (SEM).
Document ID
20040089339
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Duke, J. (University of Texas Dental Branch Houston 77225, United States)
Janer, L.
Campbell, M.
Date Acquired
August 21, 2013
Publication Date
January 1, 1985
Publication Information
Publication: Journal of electron microscopy technique
Volume: 2
ISSN: 0741-0581
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Plant Biology