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Simultaneous concentration and purification through gradient deformation chromatographyMobile-phase additives, commonly used to modulate absorbate retention in gradient elution chromatography, are usually assumed to be either linearly retained or unretained. Previous theoretical work from our laboratory has shown that these modulators, such as salts in ion-exchange and hydrophobic interaction chromatography and organic modifiers in reversed-phase chromatography, can absorb nonlinearly, giving rise to gradient deformation. Consequently, adsorbate peaks that elute in the vicinity of the head of the deformed gradient may exhibit unusual shapes, form shoulders, and/or be concentrated. These effects for a reversed-phase sorbent with aqueous acetonitrile (ACN) as the modulator are verified experimentally. Gradient deformation is demonstrated experimentally and agrees with simulations based on ACN isotherm parameters that are independently determined from batch equilibrium studies using the layer model. Unusual absorbate peak shapes were found experimentally for single-component injections of phenylalanine, similar to those calculated by the simulations. A binary mixture of tryptophan and phenylalanine is used to demonstrate simultaneous concentration and separation, again in agreement with simulations. The possibility of gradient deformation in ion-exchange and hydrophobic interaction chromatography is discussed.
Document ID
20040089497
Acquisition Source
Headquarters
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Velayudhan, A.
(Purdue University West Lafayette, IN 47907, United States)
Hendrickson, R. L.
Ladisch, M. R.
Mitchell, C. A.
Date Acquired
August 21, 2013
Publication Date
May 1, 1995
Publication Information
Publication: AIChE journal. American Institute of Chemical Engineers
Volume: 41
Issue: 5
ISSN: 0001-1541
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAGW-2329
CONTRACT_GRANT: BCS-8912150
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Number 93-10
NASA Discipline Life Support Systems
NASA Program NSCORT
Non-NASA Center

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