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A novel directly coupled gradostatThe original bidirectional compound chemostat (gradostat) described by Lovitt and Wimpenny has been simplified by making a more compact apparatus in which chemical gradients are established by diffusion between adjacent culture chambers. The experimental model (diffusion coupled (DC) gradostat) consisted of five chambers whose contents could be agitated by turbines rotating in the horizontal plane on a common shaft. Two biological experiments were designed to reveal the value of the DC gradostat. A methylotroph (Methylophilus methylotrophus) grown in a methanol gradient showed expected changes in cell viability as a function of position in the five vessel array. Cells of two species of photosynthetic bacteria (Rhodobacter capsulata and Rhodopseudomonas marina/agilis) with different salt sensitivities could be mixed and subsequently separated by the DC gradostat operating with a NaCl gradient of 0-3% w/v.
Document ID
20040089517
Document Type
Reprint (Version printed in journal)
Authors
Wimpenny, J. W. (University of Wales, College of Cardiff United Kingdom)
Earnshaw, R. G.
Gest, H.
Hayes, J. M.
Favinger, J. L.
Date Acquired
August 21, 2013
Publication Date
October 1, 1992
Publication Information
Publication: Journal of microbiological methods
Volume: 16
Issue: 2
ISSN: 0167-7012
Subject Category
Exobiology
Funding Number(s)
CONTRACT_GRANT: DMB-8415291
CONTRACT_GRANT: NGR15003-1118
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Exobiology
NASA Discipline Number 52-30
Non-NASA Center