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Calcium bridges are not load-bearing cell-wall bonds in Avena coleoptilesI examined the ability of frozen-thawed Avena sativa L. coleoptile sections under applied load to extend in response to the calcium chelators ethyleneglycol-bis-(beta-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA) and 2-[(2-bis-[carboxymethyl]amino-5-methylphenoxy)methyl]-6-methoxy-8-bis[car boxymethyl]aminoquinoline (Quin II). Addition of 5 mM EGTA to weakly buffered (0.1 mM, pH 6.2) solutions of 2(N-morpholino) ethanesulfonic acid (Mes) initiated rapid extension and wall acidification. When the buffer strength was increased (e.g. from 20 to 100 mM Mes, pH 6.2) EGTA did not initiate extension nor did it cause wall acidification. At 5 mM Quin II failed to stimulate cell extension or wall acidification at all buffer molarities tested (0.1 to 100 mM Mes). Both chelators rapidly and effectively removed Ca2+ from Avena sections. These data indicate that Ca2+ chelation per se does not result in loosening of Avena cells walls. Rather, EGTA promotes wall extension indirectly via wall acidification.
Document ID
20040089568
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Rayle, D. L.
(Department of Biology and Molecular Biology Institute, San Diego State University CA 92182, United States)
Date Acquired
August 21, 2013
Publication Date
January 1, 1989
Publication Information
Publication: Planta
Volume: 178
Issue: 1
ISSN: 0032-0935
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAGW2-30
Distribution Limits
Public
Copyright
Other
Keywords
NASA Program Space Biology
NASA Discipline Number 29-20
NASA Discipline Plant Biology
Non-NASA Center

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