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Latent nitrate reductase activity is associated with the plasma membrane of corn rootsLatent nitrate reductase activity (NRA) was detected in corn (Zea mays L., Golden Jubilee) root microsome fractions. Microsome-associated NRA was stimulated up to 20-fold by Triton X-100 (octylphenoxy polyethoxyethanol) whereas soluble NRA was only increased up to 1.2-fold. Microsome-associated NRA represented up to 19% of the total root NRA. Analysis of microsomal fractions by aqueous two-phase partitioning showed that the membrane-associated NRA was localized in the second upper phase (U2). Analysis with marker enzymes indicated that the U2 fraction was plasma membrane (PM). The PM-associated NRA was not removed by washing vesicles with up to 1.0 M NACl but was solubilized from the PM with 0.05% Triton X-100. In contrast, vanadate-sensitive ATPase activity was not solubilized from the PM by treatment with 0.1% Triton X-100. The results show that a protein capable of reducing nitrate is embedded in the hydrophobic region of the PM of corn roots.
Document ID
20040089592
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Ward, M. R.
(University of California Davis 95616, United States)
Grimes, H. D.
Huffaker, R. C.
Date Acquired
August 21, 2013
Publication Date
January 1, 1989
Publication Information
Publication: Planta
Volume: 177
ISSN: 0032-0935
Subject Category
Man/System Technology And Life Support
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Number 61-10
NASA Discipline Life Support Systems
Non-NASA Center
NASA Program CELSS

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