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Analysis of lipophilic pigments from a phototrophic microbial mat community by high performance liquid chromatographyAs assay for lipophilic pigments in phototrophic microbial mat communities using reverse phase-high performance liquid chromatography was developed which allows the separation of 15 carotenoids and chloropigments in a single 30 min program. Lipophilic pigments in a laminated mat from a commercial salina near Laguna Guerrero Negro, Baja California Sur, Mexico reflected their source organisms. Myxoxanthophyll, echinenone, canthaxanthin, and zeaxanthin were derived from cyanobacteria; chlorophyll c, and fucoxanthin from diatoms; chlorophyll a from cyanobacteria and diatoms; bacteriochlorophylls a and c, bacteriophaeophytin a, and gamma-carotene from Chloroflexus spp.; and beta-carotene from a variety of phototrophs. Sensitivity of detection was 0.6-6.1 ng for carotenoids and 1.7-12 ng for most chloropigments. This assay represents a significant improvement over previous analyses of lipophilic pigments in microbial mats and promises to have a wider application to other types of phototrophic communities.
Document ID
20040089602
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Palmisano, A. C.
(NASA Ames Research Center Moffett Field CA United States)
Cronin, S. E.
Des Marais, D. J.
Date Acquired
August 21, 2013
Publication Date
January 1, 1988
Publication Information
Publication: Journal of microbiological methods
Volume: 8
ISSN: 0167-7012
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Exobiology
NASA Discipline Number 52-30
NASA Center ARC

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