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Measurement of the rates of oxindole-3-acetic acid turnover, and indole-3-acetic acid oxidation in Zea mays seedlingsOxindole-3-acetic acid is the principal catabolite of indole-3-acetic acid in Zea mays seedlings. In this paper measurements of the turnover of oxindole-3-acetic acid are presented and used to calculate the rate of indole-3-acetic acid oxidation. [3H]Oxindole-3-acetic acid was applied to the endosperm of Zea mays seedlings and allowed to equilibrate for 24 h before the start of the experiment. The subsequent decrease in its specific activity was used to calculate the turnover rate. The average half-life of oxindole-3-acetic acid in the shoots was found to be 30 h while that in the kernels had an average half-life of 35h. Using previously published values of the pool sizes of oxindole-3-acetic acid in shoots and kernels from seedlings of the same age and variety, and grown under the same conditions, the rate of indole-3-acetic acid oxidation was calculated to be 1.1 pmol plant-1 h-1 in the shoots and 7.1 pmol plant-1 h-1 in the kernels.
Document ID
20040089639
Document Type
Reprint (Version printed in journal)
Authors
Nonhebel, H. M. (Michigan State University East Lansing 48824, United States)
Bandurski, R. S.
Date Acquired
August 21, 2013
Publication Date
November 1, 1986
Publication Information
Publication: Journal of experimental botany
Volume: 37
Issue: 184
ISSN: 0022-0957
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: PCM 8204017
CONTRACT_GRANT: NAGW-97
CONTRACT_GRANT: ORD 30668
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Plant Biology
Non-NASA Center
NASA Discipline Number 40-10