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Record Details

Record 96 of 2229
Spirosymplokos deltaeiberi nov. gen., nov. sp.: variable-diameter composite spirochete from microbial mats
External Online Source: doi:10.1007/BF00245307
Author and Affiliation:
Guerrero, R.(University of Barcelona, Department of Microbiology, Spain)
Ashen, J.
Sole, M.
Margulis, L.
Abstract: Large (up to 100 micrometers long), loosely coiled, free-living spirochetes with variable diameters (from 0.4 to 3 micrometers in the same cell) were seen at least 40 times between August 1990 and January 1993. These spirochetes were observed in mud water and enrichment media from highly specific habitats in intertidal evaporite flats at three disjunct localities, one in Spain and two in Mexico. All three are sites of commercial saltworks. Associated with Microcoleus chthonoplastes the large spirochetes from Spain display phototaxis and a composite organization. Shorter and smaller-diameter spirochetes are seen inside both healthy and spent periplasm of larger ones. Small spirochetes attached to large ones have been observed live. From two to twelve spirochete protoplasmic cylinders were seen inside a single common outer membrane. A distinctive granulated cytoplasm in which the granules are of similar diameter (20-32 nanometers) to that of the flagella (26 nanometers) was present. Granule diameters were measured in thin section and in negatively-stained whole-mount preparations. Based on their ultrastructure, large size, variable diameter, number of flagella (3 to 6), and phototactic behavior these unique spirochetes are formally named Spirosymplokos deltaeiberi. Under anoxic (or low oxygen) conditions they formed blooms in mixed culture in media selective for spirochetes. Cellobiose was the major carbon source in 80% seawater, the antibiotic rifampicin was added, mat from the original field site was present and tubes were incubated in the light at from 18-31 degrees C. Within 1-2 weeks populations of the large spirochete developed at 25 degrees C but they could not be transferred to fresh medium.
Publication Date: Jan 01, 1993
Document ID:
20040089685
(Acquired Sep 07, 2004)
Subject Category: EXOBIOLOGY
Document Type: Journal Article
Publication Information: Archives of microbiology (ISSN 0302-8933); Volume 160; 461-70
Publisher Information: Germany
Contract/Grant/Task Num: NGR0-04025; MAR 91-0874
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: BACTERIA; CLASSIFICATIONS; MICROORGANISMS; SEDIMENTS; ELECTRON MICROSCOPY; EXOBIOLOGY; MEXICO; MICROBIOLOGY; SEA WATER; SPAIN
Other Descriptors: GEOLOGIC SEDIMENTS/MICROBIOLOGY; SPIROCHAETALES/CLASSIFICATION/PHYSIOLOGY/ULTRASTRUCTURE; ENVIRONMENTAL MICROBIOLOGY; MEXICO; MICROSCOPY, ELECTRON; SEAWATER; SPAIN; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S; WATER MICROBIOLOGY; NASA DISCIPLINE EXOBIOLOGY; NON-NASA CENTER
Availability Source: Other Sources
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