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Dynamic cell culture system: a new cell cultivation instrument for biological experiments in spaceThe prototype of a miniaturized cell cultivation instrument for animal cell culture experiments aboard Spacelab is presented (Dynamic cell culture system: DCCS). The cell chamber is completely filled and has a working volume of 200 microliters. Medium exchange is achieved with a self-powered osmotic pump (flowrate 1 microliter h-1). The reservoir volume of culture medium is 230 microliters. The system is neither mechanically stirred nor equipped with sensors. Hamster kidney (Hak) cells growing on Cytodex 3 microcarriers were used to test the biological performance of the DCCS. Growth characteristics in the DCCS, as judged by maximal cell density, glucose consumption, lactic acid secretion and pH, were similar to those in cell culture tubes.
Document ID
20040089807
Document Type
Reprint (Version printed in journal)
Authors
Gmunder, F. K. (ETH-Zurich Switzerland)
Nordau, C. G.
Tschopp, A.
Huber, B.
Cogoli, A.
Date Acquired
August 21, 2013
Publication Date
January 1, 1988
Publication Information
Publication: Journal of biotechnology
Volume: 7
ISSN: 0168-1656
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: 3.382-0.82
Distribution Limits
Public
Copyright
Other
Keywords
NASA Program Flight
NASA Discipline Cell Biology
NASA Discipline Number 00-00
Non-NASA Center