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Protection of wheat against leaf and stem rust and powdery mildew diseases by inhibition of polyamine metabolismIn higher plants, polyamines arise from arginine by one of two pathways: via ornithine and ornithine decarboxylase or via agmatine and arginine decarboxylase but in fungi, only the ornithine decarboxylase pathway is present. Since polyamines are required for normal growth of microorganisms and plants and since the ornithine pathway can be irreversibly blocked by alpha-difluoromethylornithine (DFMO) which has no effect on arginine decarboxylase, fungal infection of green plants might be controlled by the site-directed use of such a specific metabolic inhibitor. DFMO at relatively low concentrations provided effective control of the three biotrophic fungal pathogens studied, Puccinia recondita (leaf rust), P. graminis f. sp. tritici (stem rust), and Erysiphe graminis (powdery mildew) on wheat (Triticum aestivum L.) Effective control of infection by leaf or stem rust fungi was obtained with sprays of DFMO that ranged from about 0.01 to 0.20 mM in experiments where the inhibitor was applied after spore inoculation. The powdery mildew fungus was somewhat more tolerant of DFMO, but good control of the pathogen was obtained at less than 1.0 mM. In general, application of DFMO after spore inoculation was more effective than application before inoculation. Less control was obtained following treatment with alpha-difluoromethylarginine (DFMA) but the relatively high degree of control obtained raises the possibility of a DFMA to DFMO conversion by arginase.
Document ID
20040089819
Document Type
Reprint (Version printed in journal)
Authors
Weinstein, L. H. (Boyce Thompson Institute Ithaca, NY 14853, United States)
Osmeloski, J. F.
Wettlaufer, S. H.
Galston, A. W.
Date Acquired
August 21, 2013
Publication Date
January 1, 1987
Publication Information
Publication: Plant science (Shannon, Ireland)
Volume: 51
ISSN: 0168-9452
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Number 40-10
Non-NASA Center
NASA Discipline Plant Biology
NASA Program Space Biology