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In vitro and in vivo protein phosphorylation in Avena sativa L. coleoptiles: effects of Ca2+, calmodulin antagonists, and auxinIn vitro and in vivo protein phosphorylations in oat (Avena sativa L.) coleoptile segments were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and by two-dimensional gel electrophoresis. In vitro phosphorylation of several polypeptides was distinctly promoted at 1 to 15 micromolar free Ca2+ concentrations. Ca2(+)-stimulated phosphorylation was markedly reduced by trifluoperazine, chlorpromazine, and naphthalene sulfonamide (W7). Two polypeptides were phosphorylated both under in vitro and in vivo conditions, but the patterns of phosphorylation of several other polypeptides were different under the two conditions indicating that the in vivo phosphorylation pattern of proteins is not truly reflected by in vitro phosphorylation studies. Trifluoperazine, W7, or ethylene glycol-bis-(beta-aminoethyl ether)-N,N'-tetraacetic acid (EGTA) + calcium ionophore A23187 treatments resulted in reduced levels of in vivo protein phosphorylation of both control and auxin-treated coleoptile segments. Analysis by two-dimensional electrophoresis following in vivo phosphorylation revealed auxin-dependent changes of certain polypeptides. A general inhibition of phosphorylation by calmodulin antagonists suggested that both control and auxin-treated coleoptiles exhibited Ca2+, and calmodulin-dependent protein phosphorylation in vivo.
Document ID
20040089820
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Veluthambi, K.
(Washington State University Pullman 99164-6414, United States)
Poovaiah, B. W.
Date Acquired
August 21, 2013
Publication Date
July 1, 1986
Publication Information
Publication: Plant physiology
Volume: 81
Issue: 3
ISSN: 0032-0889
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: DCB-8502215
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Plant Biology
NASA Program Space Biology
Non-NASA Center
NASA Discipline Number 40-30

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