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Transport and metabolism of indole-3-acetyl-myo-inositol-galactoside in seedlings of Zea maysIndole-3-acetyl-myo-inositol galactoside labeled with 3H in the indole and 14C in the galactose moieties was applied to kernels of 5 day old germinating seedlings of Zea mays. Indole-3-acetyl-myo-inositol galactoside was not transported into either the shoot or root tissue as the intact molecule but was instead hydrolyzed to yield [3H]indole-3-acetyl-myo-inositol and [3H]indole-3-acetic acid which were then transported to the shoot with little radioactivity going to the root. With certain assumption concerning the equilibration of applied [3H]indole-3-acetyl-myo-inositol-[U-14C]galactose with the endogenous pool, it may be concluded that indole-3-acetyl-myo-inositol galactoside in the endosperm supplies about 2 picomoles per plant per hour of indole-3-acetyl-myo-inositol and 1 picomole per plant per hour of indole-3-acetic acid to the shoot and thus is comparable to indole-3-acetyl-myo-inositol as a source of indole-acetic acid for the shoot. Quantitative estimates of the amount of galactose in the kernels suggest that [3H]indole-3-acetyl-myo-inositol-[14C]galactose is hydrolyzed after the compound leaves the endosperm but before it reaches the shoot. In addition, [3H]indole-3-acetyl-myo-inositol-[14C]galactose supplies appreciable amounts of 14C to the shoot and both 14C and 3H to an uncharacterized insoluble fraction of the endosperm.
Document ID
20040089861
Document Type
Reprint (Version printed in journal)
Authors
Komoszynski, M. (Michigan State University East Lansing 48824-1312, United States)
Bandurski, R. S.
Date Acquired
August 21, 2013
Publication Date
January 1, 1986
Publication Information
Publication: Plant physiology
Volume: 80
ISSN: 0032-0889
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAGW-97
CONTRACT_GRANT: PCM-8204017
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Number 40-30
NASA Program Space Biology
Non-NASA Center
NASA Discipline Plant Biology