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Early quantitative method for measuring germination in non-green spores of Dryopteris paleacea using an epifluorescence-microscope techniqueA method is described to determine germination by blue-light excited red fluorescence in the positively photoblastic spores of Dryopteris paleacea Sw. This fluorescence is due to chlorophyll as evidenced from 1) a fluorescence-emission spectrum in vivo, where a bright fluorescence around 675 nm is obtained only in red light (R)-irradiated spores and 2) in vitro measurements with acetone extracts prepared from homogenized spores. Significant amounts of chlorophyll can be found only in R-treated spores; this chlorophyll exhibits an emission band around 668 nm, when irradiated with 430 nm light at 21 degrees C. Compared to other criteria for germination, such as swelling of the cell, coat splitting, greening, and rhizoid formation, which require longer periods after induction for their expression, chlorophyll fluorescence can be used to quantify germination after two days. This result is confirmed by fluence-response curves for R-induced spore germination; the same relationship between applied R and germination is obtained by the evaluation with the epifluorescence method 2 days after the light treatment as compared with the evaluation with bright-field microscopy 5 days after the inducing R. Using this technique we show for the first time that Ca2+ contributes to the signal-transduction chain in phytochrome-mediated chlorophyll synthesis in spores of Dryopteris paleacea.
Document ID
Document Type
Reprint (Version printed in journal)
Scheuerlein, R. (Universitat Erlangen-Nurnberg Erlangen, FRG)
Wayne, R.
Roux, S. J.
Date Acquired
August 21, 2013
Publication Date
January 1, 1988
Publication Information
Publication: Physiologia plantarum
Volume: 73
Issue: 4
ISSN: 0031-9317
Subject Category
Life Sciences (General)
Funding Number(s)
Distribution Limits
NASA Discipline Plant Biology
NASA Discipline Number 40-10
Non-NASA Center
NASA Program Space Biology