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Record 32 of 381
Calcium-regulated in vivo protein phosphorylation in Zea mays L. root tips
Author and Affiliation:
Raghothama, K. G.(Washington State University, Department of Horticulture and Landscape Architecture, Pullman 99164-6414, United States)
Reddy, A. S.
Friedmann, M.
Poovaiah, B. W.
Abstract: Calcium dependent protein phosphorylation was studied in corn (Zea mays L.) root tips. Prior to in vivo protein phosphorylation experiments, the effect of calcium, ethyleneglycol-bis-(beta-aminoethyl ether)-N-N' -tetraacetic acid (EGTA) and calcium ionophore (A-23187) on phosphorus uptake was studied. Calcium increased phosphorus uptake, whereas EGTA and A-23187 decreased it. Consequently, phosphorus concentration in the media was adjusted so as to attain similar uptake in different treatments. Phosphoproteins were analyzed by two-dimensional gel electrophoresis. Distinct changes in phosphorylation were observed following altered calcium levels. Calcium depletion in root tips with EGTA and A-23187 decreased protein phosphorylation. However, replenishment of calcium following EGTA and ionophore pretreatment enhanced phosphorylation of proteins. Preloading of the root tips with 32P in the presence of EGTA and A-23187 followed by a ten minute calcium treatment, resulted in increased phosphorylation indicating the involvement of calcium, calcium and calmodulin-dependent kinases. Calmodulin antagonist W-7 was effective in inhibiting calcium-promoted phosphorylation. These studies suggest a physiological role for calcium-dependent phosphorylation in calcium-mediated processes in plants.
Publication Date: Jan 01, 1987
Document ID:
20040089871
(Acquired Sep 07, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Plant physiology (ISSN 0032-0889); Volume 83; 1008-13
Publisher Information: United States
Contract/Grant/Task Num: DCB-8502215; NAG10-0032
Financial Sponsor: NASA Kennedy Space Center; Cocoa Beach FL United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: CALCIUM; CORN; IN VIVO METHODS AND TESTS; METABOLISM; PHOSPHORUS; PHOSPHORYLATION; PLANT ROOTS; PLANTS (BOTANY); PROTEINS; CALMODULIN; CHELATION; EXOBIOLOGY; PHARMACOLOGY
Other Descriptors: CALCIUM/ANTAGONISTS & INHIBITORS/METABOLISM/PHARMACOLOGY/PHYSIOLOGY; PHOSPHORUS/PHARMACOKINETICS; PLANT PROTEINS/CHEMISTRY/METABOLISM/PHYSIOLOGY; PLANT ROOTS/DRUG EFFECTS/METABOLISM/PHYSIOLOGY; ZEA MAYS/DRUG EFFECTS/METABOLISM/PHYSIOLOGY; CALCIMYCIN/PHARMACOLOGY; CALMODULIN/ANTAGONISTS & INHIBITORS; CHELATING AGENTS/PHARMACOLOGY; EGTAZIC ACID/PHARMACOLOGY; IONOPHORES/PHARMACOLOGY; PHOSPHORYLATION; SULFONAMIDES/PHARMACOLOGY; SUPPORT, U.S. GOV'T, NON-P.H.S; SUPPORT, U.S. GOV'T, P.H.S; NASA DISCIPLINE NUMBER 40-30; NASA DISCIPLINE PLANT BIOLOGY; NASA PROGRAM SPACE BIOLOGY; NON-NASA CENTER
Availability Source: Other Sources
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