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Effects of murine leukemia virus env gene proteins on macrophage-mediated cytotoxicity in vitroF5b Tumor cells were incubated with concentrated culture supernatants taken from cells resistant (F5m) or sensitive (F5b) to contact-dependent macrophage cytotoxicity. Macrophage cell line B6MP102 and murine peritoneal macrophages killed targets incubated with supernatants taken from sensitive cells but poorly killed cells incubated in supernatants isolated from resistant cells. Membranes from cells resistant to macrophage killing, F5m, were fused into F5b cells. The fused F5b cells were killed significantly less than F5b cells fused with F5b cell membranes or untreated F5b cells. The decreased killing of F5b cells corresponded to increased concentrations of gp70(a) molecules on F5b cells. Affinity purified gp70(a) was added to cytotoxicity assays but failed to inhibit macrophage cytotoxicity. P15E molecules were detectable on both F5b and F5m cells. In addition, a synthetic peptide found to exhibit the inhibitory properties of p15E was added to cytotoxicity assays. P15E synthetic peptide also did not inhibit macrophage cytotoxicity. Therefore, env gene proteins of murine leukemia virus do not appear responsible for inducing tumor cell resistance to activated macrophage contact-dependent cytotoxicity.
Document ID
20040090048
Acquisition Source
Headquarters
Document Type
Reprint (Version printed in journal)
Authors
Chapes, S. K.
(Kansas State University Manhattan 66506, United States)
Takemoto, L. J.
Spooner, B. S.
Date Acquired
August 21, 2013
Publication Date
January 1, 1991
Publication Information
Publication: Life science advances
Volume: 10
ISSN: 0255-6642
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: DAMD17-89-Z-9039
CONTRACT_GRANT: NAGW-1197
CONTRACT_GRANT: EY 02932
Distribution Limits
Public
Copyright
Other
Keywords
NASA Program NSCORT
Non-NASA Center
NASA Discipline Number 93-10
NASA Discipline Cell Biology

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