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Growth and morphogenesis of embryonic mouse organs on non-coated and extracellular matrix-coated Biopore membraneEmbryonic mouse salivary glands, pancreata, and kidneys were isolated from embryos of appropriate gestational age by microdissection, and were cultured on Biopore membrane either non-coated or coated with type I collagen or Matrigel. As expected, use of Biopore membrane allowed high quality photomicroscopy of the living organs. In all organs extensive mesenchymal spreading was observed in the presence of type I collagen or Matrigel. However, differences were noted in the effects of extracellular matrix (ECM) coatings on epithelial growth and morphogenesis: salivary glands were minimally affected, pancreas morphogenesis was adversely affected, and kidney growth and branching apparently was enhanced. It is suggested that these differences in behaviour reflect differences in the strength of interactions between the mesenchymal cells and their surrounding endogenous matrix, compared to the exogenous ECM macromolecules. This method will be useful for culture of these and other embryonic organs. In particular, culture of kidney rudiments on ECM-coated Biopore offers a great improvement over previously used methods which do not allow morphogenesis to be followed in vitro.
Document ID
20040090094
Document Type
Reprint (Version printed in journal)
Authors
Hardman, P.
(Kansas State University Manhattan 66506-4904, United States)
Klement, B. J.
Spooner, B. S.
Date Acquired
August 21, 2013
Publication Date
January 1, 1993
Publication Information
Publication: Development, growth & differentiation
Volume: 35
Issue: 6
ISSN: 0012-1592
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAGW-2328
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Number 93-10
NASA Discipline Developmental Biology
NASA Program NSCORT
Non-NASA Center
NASA Discipline Cell Biology
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