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The role of wall calcium in the extension of cell walls of soybean hypocotylsCalcium crosslinks are load-bearing bonds in soybean (Glycine max (L.) Merr.) hypocotyl cell walls, but they are not the same load-bearing bonds that are broken during acid-mediated cell elongation. This conclusion is reached by studying the relationship between wall calcium, pH and the facilitated creep of frozen-thawed soybean hypocotyl sections. Supporting data include the following observations: 1) 2-[(2-bis-[carboxymethyl]amino-5-methylphenoxy)methyl]-6-methoxy-8-bis[car boxymethyl]aminoquinoline (Quin 2) and ethylene glycol-bis(2-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) caused only limited facilitated creep as compared with acid, despite removal of comparable or larger amounts of wall calcium; 2) the pH-response curves for calcium removal and acid-facilitated creep were different; 3) reversible acid-extension occurred even after removal of almost all wall calcium with Quin 2; and 4) growth of abraded sections did not involve a proportional loss of wall calcium. Removal of wall calcium, however, increased the capacity of the walls to undergo acid-facilitated creep. These data indicate that breakage of calcium crosslinks is not a major mechanism of cell-wall loosening in soybean hypocotyl tissues.
Document ID
20040090109
Document Type
Reprint (Version printed in journal)
Authors
Virk, S. S.
(University of Washington Seattle 98195, United States)
Cleland, R. E.
Date Acquired
August 21, 2013
Publication Date
January 1, 1990
Publication Information
Publication: Planta
Volume: 182
ISSN: 0032-0935
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: DE-FG06-88ER13830
CONTRACT_GRANT: NAGW-1394
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Plant Biology
NASA Discipline Number 29-20
NASA Program Space Biology
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