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Novel use of positively charged nylon transfer membranes for trapping indoleacetic acid or other small anions during efflux from plant tissuesPositively charged nylon blotting membranes were used as an anion binding medium to trap [14C]indoleactic acid (IAA) as it exited cells at the basal ends of Coleus blumei L. stem and Zea mays L. coleoptile segments. Autoradiography was used to visualize where the [14C] that moved out of the cut ends was localized on the nylon membrane. Diffusion of [14C]IAA from the initial point of contact with the nylon membrane was minimal. Comparison of the autoradiograms with anatomical tissue prints of the cut ends of the segments was used to determine what tissues participate in IAA movement. The results of these initial studies were consistent with other reports suggesting that [14C]IAA movement was primarily associated with vascular tissues in both C. blumei stems and corn coleoptiles, but the resolution was not sufficient to identify which vascular tissues were involved in IAA transport. With further refinements, this technique could also be used for studying the movement of other small charged molecules through plant tissues.
Document ID
20040090144
Document Type
Reprint (Version printed in journal)
Authors
Evans, M. L. (Ohio State University Columbus, United States)
Hangarter, R. P.
Date Acquired
August 21, 2013
Publication Date
January 1, 1993
Publication Information
Publication: Plant physiology and biochemistry : PPB / Societe francaise de physiologie vegetale
Volume: 31
Issue: 2
ISSN: 0981-9428
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAGW-297
CONTRACT_GRANT: DCB-9106697
CONTRACT_GRANT: DCB-9105807
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Plant Biology
Non-NASA Center
NASA Program Space Biology
NASA Discipline Number 45-10