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Characterization of photosystem 1 chlorophyll a/b-binding apoprotein accumulation in developing soybean using type-specific antibodiesThe structure and supramolecular assembly of the soybean photosystem 1 (PS 1) chlorophyll a/b-binding antenna (LHC 1) was examined. We identified the subunit composition of LHC 1 in soybean and followed the accumulation of individual subunits during light-induced assembly. We observed four LHC 1 subunits, at 23, 22, 21 and 20.5 kDa, obtained partial sequence information by amino-terminal sequence analysis, and classified the 20.5, 22, and 21 kDa subunits as being encoded by type I, II, and IV chlorophyll a/b binding protein genes, respectively. Antisera against LHC 1 subunits were used to follow the accumulation of individual subunits during the light-initiated transition from etioplast to chloroplast. Several points are noteworthy. First, monospecific antibody against the 22 kDa subunit decorated a 25 kDa peptide in etiolated tissue, which declined during maturation. This decline correlated with the light-induced appearance of mature 22 kDa peptide, suggesting a precursor/product relationship. Second, the same antibody identified a 22 kDa protein in mature corn, but not a larger band in etiolated corn, suggesting that LHC 1 accumulation is regulated differently between species before the onset of chlorophyll biosynthesis. Third, the mature 22 kDa subunit appeared somewhat later than the other LHC 1 peptides during greening, implying that this subunit is less intimately associated with the PS1 core than are the subunits appearing earlier in development.
Document ID
20040090218
Acquisition Source
Headquarters
Document Type
Reprint (Version printed in journal)
Authors
Henry, R. L.
(Kansas State University Manhattan, United States)
Armbrust, T.
Gallegos, G.
Guikema, J. A.
Spooner, B. S.
Date Acquired
August 21, 2013
Publication Date
January 1, 1992
Publication Information
Publication: Plant physiology and biochemistry : PPB / Societe francaise de physiologie vegetale
Volume: 30
Issue: 3
ISSN: 0981-9428
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAGW-1197
CONTRACT_GRANT: NAGW-2328
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Developmental Biology
NASA Discipline Number 93-10
NASA Program NSCORT
NASA Discipline Cell Biology

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