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Ferredoxin and ferredoxin-NADP reductase from photosynthetic and nonphotosynthetic tissues of tomatoFerredoxin and ferredoxin-NADP+ oxidoreductase (FNR) were purified from leaves, roots, and red and green pericarp of tomato (Lycopersicon esculentum, cv VFNT and cv Momotaro). Four different ferredoxins were identified on the basis of N-terminal amino acid sequence and charge. Ferredoxins I and II were the most prevalent forms in leaves and green pericarp, and ferredoxin III was the most prevalent in roots. Red pericarp of the VFNT cv yielded variable amounts of ferredoxins II and III plus a unique form, ferredoxin IV. Red pericarp of the Momotaro cv contained ferredoxins I, II, and IV. This represents the first demonstration of ferredoxin in a chromoplast-containing tissue. There were no major differences among the tomato ferredoxins in absorption spectrum or cytochrome c reduction activity. Two forms of FNR were present in tomato as judged by anion exchange chromatography and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. FNR II had a lower apparent relative molecular weight, a slightly altered absorption spectrum, and a lower specific activity for cytochrome c reduction than FNR I. FNR II could be a partially degraded form of FNR I. The FNRs from the different tissues of tomato plants all showed diaphorase activity, with FNR II being more active than FNR I. The presence of ferredoxin and FNR in heterotrophic tissues of tomato is consistent with the existence of a nonphotosynthetic ferredoxin/FNR redox pathway to support the function of ferredoxin-dependent enzymes.
Document ID
Document Type
Reprint (Version printed in journal)
Green, L. S. (University of California Berkeley 94720, United States)
Yee, B. C.
Buchanan, B. B.
Kamide, K.
Sanada, Y.
Wada, K.
Date Acquired
August 21, 2013
Publication Date
January 1, 1991
Publication Information
Publication: Plant physiology
Volume: 96
ISSN: 0032-0889
Subject Category
Life Sciences (General)
Funding Number(s)
Distribution Limits
NASA Discipline Number 52-30
NASA Program Exobiology
Non-NASA Center
NASA Discipline Exobiology