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Isatin as an auxin source favoring floral and vegetative shoot regeneration from calli produced by thin layer explants of tomato pedicelThin layer explants taken from the pedicels and peduncles of flowering tomato plants yielded calli with great organogenetic potential. Of the 15 cultivars tested, 7 regenerated roots, shoots and eventually entire fruit-bearing plants. Calli grown on modified Murashige-Skoog medium responded to varied auxins and cytokinins with different morphogenetic patterns. Thus, naphthaleneacetic acid yielded root-producing calli, while the auxin precursor isatin (indole 2,3-dione) caused the production of calli with vegetative and floral shoots, rarely yielding roots. This may be related to isatin's slow, steady conversion to an active auxin (Plant Physiol 41:1485-1488, 1966) in contrast with naphthaleneacetic acid's immediate presentation of a high level of active auxin. The highest incidence of vegetative shoot (100%) and flower (50%) formation was obtained with 10 micromoles isatin and 3 micromoles zeatin. A few of the flowers developed into ripe fruits. The high frequency of induction of vegetative shoots and flowers before roots with isatin suggests its utility in micropropagation from plant tissue cultures.
Document ID
Document Type
Reprint (Version printed in journal)
External Source(s)
Applewhite, P. B.
(Yale University New Haven, CT 06570, United States)
K-Sawhney, R.
Galston, A. W.
Date Acquired
August 21, 2013
Publication Date
January 1, 1994
Publication Information
Publication: Plant growth regulation
Volume: 15
ISSN: 0167-6903
Subject Category
Man/System Technology And Life Support
Distribution Limits
NASA Discipline Life Support Systems
Non-NASA Center
NASA Discipline Number 61-10
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