NASA Logo, External Link
Facebook icon, External Link to NASA STI page on Facebook Twitter icon, External Link to NASA STI on Twitter YouTube icon, External Link to NASA STI Channel on YouTube RSS icon, External Link to New NASA STI RSS Feed AddThis share icon
 

Record Details

Record 3 of 5862
Antipeptide antibodies that can distinguish specific subunit polypeptides of glutamine synthetase from bean (Phaseolus vulgaris L.)
Author and Affiliation:
Cai, X.(Kansas State University, Division of Biology, Manhattan 66506, United States)
Henry, R. L.
Takemoto, L. J.
Guikema, J. A.
Wong, P. P.
Spooner, B. S. [Principal Investigator]
Abstract: The amino acid sequences of the beta and gamma subunit polypeptides of glutamine synthetase from bean (Phaseolus vulgaris L.) root nodules are very similar. However, there are small regions within the sequences that are significantly different between the two polypeptides. The sequences between amino acids 2 and 9 and between 264 and 274 are examples. Three peptides (gamma 2-9, gamma 264-274, and beta 264-274) corresponding to these sequences were synthesized. Antibodies against these peptides were raised in rabbits and purified with corresponding peptide-Sepharose affinity chromatography. Western blot analysis of polyacrylamide gel electrophoresis of bean nodule proteins demonstrated that the anti-beta 264-274 antibodies reacted specifically with the beta polypeptide and the anti-gamma 264-274 and anti-gamma 2-9 antibodies reacted specifically with the gamma polypeptide of the native and denatured glutamine synthetase. These results showed the feasibility of using synthetic peptides in developing antibodies that are capable of distinguishing proteins with similar primary structures.
Publication Date: Jan 01, 1992
Document ID:
20040090274
(Acquired Sep 07, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Plant physiology (ISSN 0032-0889); Volume 98; 402-5
Publisher Information: United States
Contract/Grant/Task Num: 8837120-3891; NAGW-1197
Financial Sponsor: NASA; Washington DC United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: ANTIBODIES; ENZYMES; ENZYMOLOGY; GLUTAMINE; LEGUMINOUS PLANTS; PEPTIDES; PLANTS (BOTANY); POLYPEPTIDES; AMINO ACIDS; PLANT ROOTS
Other Descriptors: ANTIBODY SPECIFICITY; FABACEAE/ENZYMOLOGY/IMMUNOLOGY; GLUTAMATE-AMMONIA LIGASE/ANALYSIS/CHEMISTRY; PEPTIDES/ANALYSIS/CHEMICAL SYNTHESIS/CHEMISTRY; PLANTS, MEDICINAL; AMINO ACID SEQUENCE; ANTIBODIES; ISOENZYMES; PLANT ROOTS/ENZYMOLOGY/IMMUNOLOGY; SUPPORT, U.S. GOV'T, NON-P.H.S; SUPPORT, U.S. GOV'T, P.H.S; NASA DISCIPLINE CELL BIOLOGY; NASA DISCIPLINE NUMBER 93-10; NASA PROGRAM NSCORT; NON-NASA CENTER
Availability Source: Other Sources
› Back to Top
Find Similar Records
NASA Logo, External Link
NASA Official: Gerald Steeman
Site Curator: STI Program
Last Modified: August 23, 2011
Contact Us