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Green and non-green callus induction from excised rice (Oryza sativa) embryos: effects of exogenous plant growth regulatorsCalli were induced either from excised rice embryos or from whole seeds in the presence of 1 to 5 mg l-1 NAA. After 12 days of culture, calli were induced only from excised rice embryos. We found that excised embryos accumulated NAA up to 6 times higher concentration than did whole seeds. In the presence of 1 to 5 mg l-1 NAA and 2 to 10 mg l-1 kinetin, chlorophyllous calli were induced from excised rice embryos. Chlorophyll contents in the callus tissue increased with increasing kinetin concentration while percent callus induction decreased. The total chlorophyll content was linearly correlated with the ratio of kinetin to NAA in the medium.
Document ID
20040090309
Acquisition Source
Kennedy Space Center
Document Type
Reprint (Version printed in journal)
Authors
Kim, D.
(Univ. of Michigan Ann Arbor 48109)
Brock, T. G.
Kaufman, P. B.
Date Acquired
August 21, 2013
Publication Date
January 1, 1992
Publication Information
Publication: Quarterly (Plant Growth Regulator Society of America)
Volume: 20
Issue: 4
ISSN: 1042-3524
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAG10-0069
CONTRACT_GRANT: NAGW-1600
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Number 40-50
NASA Discipline Plant Biology
Non-NASA Center
NASA Program Space Biology

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