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Iodine susceptibility of pseudomonads grown attached to stainless steel surfacesPseudomonads were adapted to grow in phosphate-buffered water and on stainless steel surfaces to study the iodine sensitivity of attached and planktonic cells. Cultures adapted to low nutrient growth were incubated at room temperature in a circulating reactor system with stainless steel coupons to allow biofilm formation on the metal surfaces. In some experiments, the reactor was partially emptied and refilled with buffer at each sampling time to simulate a "fill-and-draw" water system. Biofilms of attached bacteria, resuspended biofilm bacteria, and reactor suspension, were exposed to 1 mg l-1 iodine for 2 min. Attached bacterial populations which established on coupons within 3 to 5 days displayed a significant increase in resistance to iodine. Increased resistance was also observed for resuspended cells from the biofilm and planktonic bacteria in the system suspension. Generally, intact biofilms and resuspended biofilm cells were most resistant, followed by planktonic bacteria and phosphate buffer cultures. Thus, biofilm formation on stainless steel surfaces within water systems can result in significantly increased disinfection resistance of commonly-occurring water-borne bacteria that may enhance their ability to colonise water treatment and distribution systems.
Document ID
Document Type
Reprint (Version printed in journal)
Pyle, B. H.
(Montana State University Bozeman 59717)
McFeters, G. A.
Date Acquired
August 21, 2013
Publication Date
January 1, 1990
Publication Information
Publication: Biofouling
Volume: 2
ISSN: 0892-7014
Subject Category
Life Sciences (General)
Funding Number(s)
Distribution Limits
NASA Program Environmental Health
NASA Discipline Number 04-10
NASA Discipline Environmental Health
Non-NASA Center

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