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Record Details

Record 1 of 4103
Evidence for a plasma-membrane-bound nitrate reductase involved in nitrate uptake of Chlorella sorokiniana
External Online Source: doi:10.1007/BF00392522
Author and Affiliation:
Tischner, R.(Universitat Gottingen, Pharmaphysiologisches Institut, Federal Republic of Germany)
Ward, M. R.
Huffaker, R. C.
Abstract: Anti-nitrate-reductase (NR) immunoglobulin-G (IgG) fragments inhibited nitrate uptake into Chlorella cells but had no affect on nitrate uptake. Intact anti-NR serum and preimmune IgG fragments had no affect on nitrate uptake. Membrane-associated NR was detected in plasma-membrane (PM) fractions isolated by aqueous two-phase partitioning. The PM-associated NR was not removed by sonicating PM vesicles in 500 mM NaCl and 1 mM ethylenediaminetetraacetic acid and represented up to 0.8% of the total Chlorella NR activity. The PM NR was solubilized by Triton X-100 and inactivated by Chlorella NR antiserum. Plasma-membrane NR was present in ammonium-grown Chlorella cells that completely lacked soluble NR activity. The subunit sizes of the PM and soluble NRs were 60 and 95 kDa, respectively, as determined by sodium-dodecyl-sulfate electrophoresis and western blotting.
Publication Date: Jan 01, 1989
Document ID:
20040090331
(Acquired Sep 07, 2004)
Subject Category: MAN/SYSTEM TECHNOLOGY AND LIFE SUPPORT
Document Type: Journal Article
Publication Information: Planta; p. 19-24; (ISSN 0032-0935); Volume 178
Publisher Information: Germany
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: CHLORELLA; MEMBRANES; METABOLISM; NITRATES; PLASMAS (PHYSICS); ANTIBODIES; ANTISERUMS; CELL MEMBRANES (BIOLOGY); CLOSED ECOLOGICAL SYSTEMS; CYTOLOGY; GLOBULINS; IMMUNOLOGY; LIFE SUPPORT SYSTEMS; OXIDATION-REDUCTION REACTIONS; SOLUBILITY
Other Descriptors: CHLORELLA/CYTOLOGY/METABOLISM/PHYSIOLOGY/ULTRASTRUCTURE; NITRATE REDUCTASES/ANALYSIS/ANTAGONISTS & INHIBITORS/PHYSIOLOGY; NITRATES/PHARMACOKINETICS; BIOLOGICAL TRANSPORT; CELL MEMBRANE/ENZYMOLOGY; CYTOSOL/ENZYMOLOGY; IMMUNE SERA/IMMUNOLOGY/PHARMACOLOGY; IMMUNOGLOBULIN G/PHARMACOLOGY; OXIDATION-REDUCTION; SOLUBILITY; NASA DISCIPLINE LIFE SUPPORT SYSTEMS; NASA DISCIPLINE NUMBER 61-10; NASA PROGRAM CELSS; NON-NASA CENTER
Availability Source: Other Sources
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