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Detergent solubilization of the EGF receptor from A431 cellsFunctional reconstitution of purified preparations of human epidermal growth factor receptor (EGFR) requires dissociation of the protein from its plasma membrane lipid environment. Solubilization of membrane proteins in this manner requires the use of detergents, which are known to disrupt plasma membrane lipid/protein interactions. We have investigated the ability of three nonionic detergents to solubilize the human EGFR selectively, and have also analyzed the effect of these various treatments on the intrinsic tyrosyl kinase activity of the receptor. The nonionic detergent known as n-octyl glucoside (n-octyl beta-D-glucopyranoside) was found to give the best combination of selectivity, yield, and maintenance of enzymatic activity of the human EGFR.
Document ID
20040090345
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Dayanidhi, R.
(Kansas State University Manhattan 66506-4901, United States)
Rintoul, D. A.
Spooner, B. S.
Date Acquired
August 21, 2013
Publication Date
April 1, 1993
Publication Information
Publication: Transactions of the Kansas Academy of Science
Volume: 96
Issue: 1-2
ISSN: 0022-8443
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Program NSCORT
NASA Discipline Number 93-10
NASA Discipline Cell Biology

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