NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Cytoskeleton-amyloplast interactions in sweet cloverThe distribution of organelles within columella cells of sweet clover was examined by transmission electron microscopy following growth under static or clinorotating conditions. A developmentally conditioned polarity was observed, with a proximal location of the nucleus and a distal accumulation of the endoplasmic reticulum. This polarity was insensitive to clinorotation. In contrast, clinorotation altered the location of amyloplasts. Application of cytoskeletal poisons (colchicine, cytochalasin D, taxol, and phalloidin), especially during clinorotation, had interesting effects on the maintenance of columella cell polarity, with a profound effect on the extent, location, and structure of the endoplasmic reticulum. The site of cytoskeletal interactions with sedimenting amyloplasts is thought to be the amyloplast envelope. An envelope fraction, having over 17 polypeptides, was isolated using immobilized antibody technology, and will provide a means of assessing the role of specific peptides in cytoskeleton/amyloplast interactions.
Document ID
20040090347
Document Type
Reprint (Version printed in journal)
Authors
Guikema, J. A. (Kansas State University Manhattan 66506-4901, United States)
Hilaire, E.
Odom, W. R.
Spooner, B. S.
Date Acquired
August 21, 2013
Publication Date
April 1, 1993
Publication Information
Publication: Transactions of the Kansas Academy of Science
Volume: 96
Issue: 2-Jan
ISSN: 0022-8443
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAGW-2328
CONTRACT_GRANT: NAGW-1197
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Number 93-10
NASA Program NSCORT
NASA Discipline Cell Biology
NASA Discipline Developmental Biology