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Exposure to Carbon Nanotube Material: Assessment of Nanotube Cytotoxicity Using Human Keratinocyte CellsCarbon nanotubes are new members of carbon allotropes similar to fullerenes and graphite. Because of their unique electrical, mechanical, and thermal properties, carbon nanotubes are important for novel applications in the electronics, aerospace, and computer industries. Exposure to graphite and carbon materials has been associated with increased incidence of skin diseases, such as carbon fiber dermatitis, hyperkeratosis, and naevi. We investigated adverse effects of single-wall carbon nanotubes (SWCNT) using a cell culture of immortalized human epidermal keratinocytes (HaCaT). After 18 h of exposure of HaCaT to SWCNT, oxidative stress and cellular toxicity were indicated by formation of free radicals, accumulation of peroxidative products, antioxidant depletion, and loss of cell viability. Exposure to SWCNT also resulted in ultrastructural and morphological changes in cultured skin cells. These data indicate that dermal exposure to unrefined SWCNT may lead to dermal toxicity due to accelerated oxidative stress in the skin of exposed workers.
Document ID
20040095257
Document Type
Reprint (Version printed in journal)
Authors
Shvedova, Anna A. (National Inst. of Occupational Safety and Health Morgantown, WV, United States)
Castranova, Vincent (National Inst. of Occupational Safety and Health Morgantown, WV, United States)
Kisin, Elena R. (National Inst. of Occupational Safety and Health Morgantown, WV, United States)
Schwegler-Berry, Diane (National Inst. of Occupational Safety and Health Morgantown, WV, United States)
Murray, Ashley R. (West Virginia Univ. Morgantown, WV, United States)
Gandelsman, Vadim Z. (Science Applications International Corp. Houston, TX, United States)
Maynard, Andrew (National Inst. of Occupational Health and Safety Cincinnati, Ohio, United States)
Baron, Paul (National Inst. of Occupational Health and Safety Cincinnati, Ohio, United States)
Date Acquired
August 21, 2013
Publication Date
October 24, 2003
Publication Information
Publication: Journal of toxicology and environmental health. Part A
Volume: 66
Issue: 20
ISSN: 1528-7394
Subject Category
Life Sciences (General)
Report/Patent Number
JSC-CN-8128
Distribution Limits
Public
Copyright
Other