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Crystallization and preliminary X-ray analysis of Der f 2, a potent allergen derived from the house dust mite (Dermatophagoides farinae)Although a number of allergens have been identified and isolated, the underlying molecular basis for the potent immune response is poorly understood. House dust mites (Dermatophagoides sp.) are ubiquitous contributors to atopy in developed countries. The rhinitis, dermatitis and asthma associated with allergic reactions to these arthropods are frequently caused by relatively small (125-129 amino acids) mite proteins of unknown biological function. Der f 2, a major allergen from the mite D. farinae, has been recombinantly expressed, characterized and crystallized. The crystals belong to the tetragonal space group I4(1)22, with unit-cell parameters a = b = 95.2, c = 103.3 A. An essentially complete (97.2%) data set has been collected to 2.4 A at a synchrotron source. Attempts to solve the crystal structure of Der f 2 by molecular replacement using the NMR coordinates for either Der f 2 or Der p 2 (the homologous protein from D. pteronyssinus) failed, but preliminary searches using the crystalline Der p 2 atomic coordinates appear to be promising.
Document ID
20040099519
Document Type
Reprint (Version printed in journal)
Authors
Roeber, Dana (NASA Marshall Space Flight Center Huntsville AL United States)
Achari, Aniruddha
Takai, Toshiro
Okumura, Yasushi
Scott, David L.
Date Acquired
August 21, 2013
Publication Date
June 1, 2003
Publication Information
Publication: Acta crystallographica. Section D, Biological crystallography
Volume: 59
Issue: Pt 6
ISSN: 0907-4449
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other