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Nitric Oxide Signaling in Hypergravity-Induced Neuronal PlasticityThe goal of this research project was to identify the neurons and circuits in the vestibular nuclei and nucleus prepositus hypoglossi that utilize nitric oxide (NO) for intercellular signaling during gravity-induced plasticity. This objective was pursued using histochemical and immunocytochemical approaches to localize NO-producing neurons and characterize the fine morphology of the cells in ground-based studies of normal rats, rats adapted to hypergravity, and rats adapted to hypergravity and then re-adapted to the 1G environment. NO-producing neurons were identified and studied using four methodologies: i) immunocytochemistry employing polyclonal antibodies directed against neuronal nitric oxide synthase (nNOS), to provide an indication of the capacity of a cell for NO production; ii) immunocytochemistry employing a monoclonal antibody directed against L-citrulline, to provide an indirect index of the enzyme's activity; iii) histochemistry based on the NADPH-diaphorase reaction, for fuI1 cytological visualization of neurons; and iv) double immunofluorescence to co-localize nNOS and L-citrulline in individual vestibular nuclei (VN) and neurons.
Document ID
Acquisition Source
Ames Research Center
Document Type
Contractor or Grantee Report
Holstein, Gay R.
(Mount Sinai School of Medicine New York, NY, United States)
Date Acquired
August 21, 2013
Publication Date
January 1, 2003
Subject Category
Aerospace Medicine
Funding Number(s)
Distribution Limits
Work of the US Gov. Public Use Permitted.
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