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Improved method for HPLC analysis of polyamines, agmatine and aromatic monoamines in plant tissueThe high performance liquid chromatographic (HPLC) method of Flores and Galston (1982 Plant Physiol 69: 701) for the separation and quantitation of benzoylated polyamines in plant tissues has been widely adopted by other workers. However, due to previously unrecognized problems associated with the derivatization of agmatine, this important intermediate in plant polyamine metabolism cannot be quantitated using this method. Also, two polyamines, putrescine and diaminopropane, also are not well resolved using this method. A simple modification of the original HPLC procedure greatly improves the separation and quantitation of these amines, and further allows the simulation analysis of phenethylamine and tyramine, which are major monoamine constituents of tobacco and other plant tissues. We have used this modified HPLC method to characterize amine titers in suspension cultured carrot (Daucas carota L.) cells and tobacco (Nicotiana tabacum L.) leaf tissues.
Document ID
Document Type
Reprint (Version printed in journal)
Slocum, R. D.
(Williams College Williamstown, Massachusetts 01267, United States)
Flores, H. E.
Galston, A. W.
Weinstein, L. H.
Date Acquired
August 21, 2013
Publication Date
January 1, 1989
Publication Information
Publication: Plant physiology
Volume: 89
ISSN: 0032-0889
Subject Category
Life Sciences (General)
Distribution Limits
Non-NASA Center
NASA Discipline Plant Biology
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